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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in many tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being described but comprehensive nucleotide sequence information is just not still obtainable.
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Blocking peptides are peptides that bind especially into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines further achievable optimization of cellular and immunoassay parameters to guarantee the very best results are...
Antibodies bound to the blocking peptide now not bind into the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to the hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E kinds a fancy with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates with the G1-S phase boundary which is degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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